Part:BBa_K216004:Experience

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Applications of BBa_K216004

As noted on the main page, this part was designed in order to allow cross-signalling from a periplasmic receptor protein to a promoter inside the cell. A periplasmic receptor protein derived from Ribose Binding Protein (such as the TNT receptors K216002 and K216003) interacts with the extracellular Trg domain of the fusion protein, and causes activation of the intracellular EnvZ sensor kinase domain, phosphorylating the cognate response regulator OmpR, which then activates promoters such as the ompC promoter (available as BBa_R0082). This allows a reporter gene to be activated by an extracellular ligand. The ability to computationally redesign Ribose Binding Protein, as reporter by Looger et al (2003)(see part design information for K216002/K216003) makes this a potentially generalisable system for making bioreporters/biosensors that detect extracellular ligands. If used in a chassis other than Escherichia coli, it would probably be necessary to also provide OmpR as a BioBrick.

User Reviews

UNIQ6216f94756f9bc14-partinfo-00000000-QINU UNIQ6216f94756f9bc14-partinfo-00000001-QINU